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Download Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals) epub

by Thomas Köhler,Dirk Laßner,Anne-Katrin Rost,Barbara Thamm,Barbara Pustowoit,Harald Remke




In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
Download Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals) epub
ISBN: 3540591923
ISBN13: 978-3540591924
Category: Science
Subcategory: Biological Sciences
Author: Thomas Köhler,Dirk Laßner,Anne-Katrin Rost,Barbara Thamm,Barbara Pustowoit,Harald Remke
Language: English
Publisher: Springer; 1995 edition (November 27, 1995)
Pages: 166 pages
ePUB size: 1334 kb
FB2 size: 1117 kb
Rating: 4.3
Votes: 562
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